V5387
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 | Immunohistochemistry Rat substantia innominata (or Ch 2) tissue stained with Rabbit Anti-Vesicular Acetylcholine Transporter (VAChT) (Cat. No. V5387). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel |
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V5387
Sigma
Anti-Vesicular Acetylcholine Transporter (VAChT) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD01634356 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminal of rat vesicular acetylcholine transporter (VAChT) (amino acids 512-530). |
| Specificity | The sequence is highly conserved in human VAChT. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~70 kDa |
| species reactivity | rat, human |
| application(s) | immunoblotting: 1:2,000 using rat brain extract |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using 4% paraformaldehyde perfusion-fixed, floating tissue sections of rat brain | |
| indirect immunofluorescence: 1:500 using NGF-treated PC12 cells | |
| Gene Information | human ... SLC18A3(6572) |
| rat ... Slc18a3(60422) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Gao, J., et al. , Orphan Glutamate Receptor delta1 Subunit Required for High-Frequency Hearing. Mol. Cell. Biol. 27, 4500-4512, (2007) Abstract |
| Jones, P., Projections from basal forebrain to prefrontal cortex comprise cholinergic, GABAergic and glutamatergic inputs to pyramidal cells or interneurons Eur. J. Neurosci. 20, 1855-1864, (2008) | |
| Jones, P., Innervation of Orexin/Hypocretin Neurons by GABAergic, Glutamatergic or Cholinergic Basal Forebrain Terminals Evidenced by Immunostaining for Presynaptic Vesicular Transporter and Postsynaptic Scaffolding Proteins J. Comp. Neurol. 499, 645-661, (2006) Abstract |
